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A chitosan-based membrane chromatography was set up by using natural chitosan/carboxymethylchitosan (CS/CMCS) blend membrane as the matrix. The dynamic adsorption property for protein (lysozyme as model protein) was detailed discussed with the change in pore size of the membrane, the flow rate and the initial concentration of the feed solution, and the layer of membrane in membrane stack. The best...
The thermally-induced conformational transitions of soybean protein films were studied as the films were heated under isothermal conditions in the range 40–100 °C. By comparing the kinetics of protein–water interactions as a function of temperature using time-resolved Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and dynamic mechanical thermal analysis (DTMA) measurements...
A macroporous amphoteric membrane was successfully prepared by solution blending of a natural polymer chitosan (CS) and its derivative carboxymethylchitosan (CMCS). The adsorption of two model proteins (ovalbumin and lysozyme) with very different pI values on this CS/CMCS blend membrane was investigated in batch systems. The results showed that both proteins could be effectively adsorbed on the membrane,...
A natural amphoteric polyelectrolyte hydrogel film was prepared by solution blending of chitosan and its derivative carboxymethylchitosan, and cross-linked with glutaraldehyde. Under electric stimulus such a hydrogel quickly bends toward one electrode, showing an electrical sensitive behavior. Because of its amphoteric nature, the hydrogel bends either toward anode (pH≤7) or cathode (pH>7), depending...
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