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An improved protocol for shoot regeneration from hairy roots transformed by Agrobacterium rhizogenes of the legume species Astragalus sinicus (Chinese milk vetch) has been developed. The A. rhizogenes strain DC-AR2 harboring the binary vector pBI121 which carries the uidAgene encoding β-glucuronidase activity and the kanamycin resistance gene nptII, was used to transform cut ends of plantlet hypocotyls...
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