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To explore the inhibition of juglone in Qinglongyi on A-549 cells in vitro, MTT assay was used. Laser confocal scanning microscope was used to observe apoptotic morphology. Changes of cell cycle are studied by flow cytometry analysis. The result of MTT assay showed that juglone had a marked growth inhibition in A-549 cells and the IC50 is respectively 3.4 × 10-5 mol/L, 1.8 × 10-5 mol/L and 2.6 × 10...
To study the effect of Saponins of asparagus on HepG2 apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (Δψm) levels. Saponins of asparagus with different concentration was treated with HepG2 at different time, MTT assay was used to detect inhibitory rate, fluorescence staining was used to observe apoptosis morphology, flow cytometry was used to detect apoptosis rate and...
To study the effect of saponins of asparagus on apoptosis and the variations of Caspase-8, 9, 3 activity in the process of asparagus induced apoptosis in HepG-2. Cell was administrated with different concentrations of asparagus at different time, the apoptosis rate was determined by FCM with AV-PI staining, the morphology was observed by electron microscopy, Caspase-8, 9, 3 activities was measured...
To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor 1(CD35) in S180 mice. Red blood cells from mice venous blood are labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Using flow cytometry, we determined the number of CR1. Using microscope, we studied the adherence between erythrocyte...
To study the effect of saponins of asparagus on apoptosis and the variations of Caspase-8, 9, 3 activity in the process of asparagus induced apoptosis in HepG-2. Cell was administrated with different concentrations of asparagus at different time, the apoptosis rate was determined by FCM with AV-PI staining, the morphology was observed by electron microscopy, Caspase-8, 9, 3 activities was measured...
To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor 1(CD35) in S180 mice. Red blood cells from mice venous blood are labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Using flow cytometry, we determined the number of CR1. Using microscope, we studied the adherence between erythrocyte...
To study the effect of Saponins of asparagus on HepG2 apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (Deltapsim) levels. Saponins of asparagus with different concentration was treated with HepG2 and SGC-7901 at different time, MTT assay was used to detect inhibitory rate, fluorescence staining was used to observe apoptosis morphology, flow cytometry was used to detect...
To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor 1(CD35) in S180 mice red blood cells from mice venous blood are labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Using flow cytometry, we determined the number of CR1. Using microscope, we studied the adherence between erythrocyte...
To explore the inhibition of juglone in Qinglongyi on A-549 cells in vitro, MTT assay was used. Laser confocal scanning microscope was used to observe apoptotic morphology. Changes of cell cycle are studied by flow cytometry analysis. The result of MTT assay showed that juglone had a marked growth inhibition in A-549 cells and the IC50 is respectively 3.4times10-5mol/L, 1.8times10-5mol/L and 2.6times10...
To study the effect of saponins of asparagus on apoptosis and the variations of Caspase-8,9,3 activity in the process of asparagus induced apoptosis in HepG-2. Cell was administrated with different concentrations of asparagus at different time, the apoptosis rate was determined by FCM with AV-PI staining, the morphology was observed by electron microscopy, Caspase-8,9,3 activities was measured by...
To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor 1(CD35) in S180 mice. Red blood cells from mice venous blood are labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Using flow cytometry, we determined the number of CR1. Using microscope, we studied the adherence between erythrocyte...
Laser scanning confoncal technology was used to study relationship between apoptosis and changes of Ca2+ induced by CSA (Capparis spinosa L. total alkaloid, CSA) on human heptocarcinoma cell HepG-2. Killing effects of CSA on human heptocarcinoma cell HepG-2 was measured by MTT method, while morphological observation on HepG-2 cells was completed by fluorescence microscope. Apoptosis of HepG-2 cells...
The mechanism and effect of the PH, drug solution concentration, intestinal segments, P-gp on Cinnamic acid absorption in rat's intestine were investigated by using in situ single-pass perfusion technique in rats. Results showed that the absorbed dose of Cinnamic acid was increased linearly and no saturation of high concentration was observed when drug concentration was in rang of 0.02-0.10 mgmiddotml...
To investigate the pharmacokinetics of loganin in rat plasma which is administered total glycoside (TG) of Jinkuishenqi pill with three different doses and the influence of verapamil. The three different doses (2.95 4.43 5.90 gmiddotkg-1)of TG alone or TG(5.90 gmiddotkg-1) in combination with verapamil (10 mgmiddotkg-1) was administered to Wistar rats. Serial blood samples of loganin were measured...
To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor 1(CD35) in S180 mice. Red blood cells from mice venous blood are labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Using flow cytometry, we determined the number of CR1. Using microscope, we studied the adherence between erythrocyte...
In order to study mitochondria signal transduction pathway of apoptosis of SGC-7901 induced by n-butanol extract from Capparis spionosa L. (CSBE), effect of apoptosis of SGC-7901 induced by CSBE was observed. In addition, the changes of initiator, effector and marker in process of mitochondria signal transduction pathway were surveyed. The inhibitory and killing effects of SGC-7901 induced by CSBE...
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