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The −1 subsite of bacterial fructansucrases (FSs) (levansucrases and inulosucrases) plays an important role in the substrate recognition, binding and catalysis. Three residues (for example W47, W118 and R193, Zymomonas mobilis levansucrase numbering) at the −1 subsite are completely conserved among FSs. Site-directed mutational analysis showed that the substitutions of the three strictly conserved...
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