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We tested the hypothesis that shedding of membrane FasL is a mechanism for downregulating FasL/Fas signaling and both membrane and soluble FasL are involved in cardiomyocyte hypoxia/ reoxygenation (H/R) injury. We examined the relative importance of mitochondrial damage and direct cleavage of the executioner caspases by activated initiator caspase-8 in the propagation of FasL/Fas signaling activated...
We explore whether antisense oligodeoxy-nucleotides (ASODN) targeting survivin gene can reverse apoptotic resistance induced by cisplatin in human lung adenocarcinoma cells. A549 / CDDP cell lines were cultured routinely with RPMI-1640 medium. Survivin ASODN mediated by cytofectin was transfected into the A549 / CDDP cells. The influence of ASODN transfection on apoptosis was determined by Diphenylamine...
We verify whether P-glycoprotein (P-gp) could induce cell resistance to apoptosis by inhibiting caspase-8 and caspase-3. Human KB cells, either drug-sensitive or with multidrug resistance (MDR) phenotype caused by Over- expression of P-gp (KBv200 cells), were treated with anti-Fas (CH-11) to induce apoptosis. Cytotoxicity was detected by MTT assay. Symptoms of cell death were assessed by morphological...
We investigate the effects of anti-Fas ribozyme on Fas expression and apoptosis in primary cultured Neonatal mouse ventricular myocytes. Neonatal mouse ventricular myocytes were isolated by using collagenase irrigation. A hammerhead ribozyme targeting the Fas mRNA was constructed, and transfected into Neonatal mouse ventricular myocytes via Effectene. Then Fas expression in Neonatal mouse ventricular...
The aim of this study was to investigate the expression of Fas and Fas ligand (FasL) and to determine the signifficance of these molecules in lung cancer cell lines. Immunoblotting, RT- PCR and flow cytometric analyses were carried out to measure the expression of Fas and FasL and to examine their interactions and e.ects on cell growth and apoptosis. Fas and FasL were co-expressed in most of the cell...
Lack of surface Fas expression is a main route for apoptotic resistance which is considered an important mechanism of tumorigenesis and tumor progression. Fas and FasL expressions in 110 non-small cell lung carcinomas (NSCLCs) were investigated to evaluate their roles in pulmonary carcinogenesis and to examine the clinicopathologic significance of Fas expression with its relationship with p53 and...
Objective To investigate the relationship between caspase-3 expression and apoptosis in the lung adenocarcinoma cell line A-549 that was transfected with the adenovirus-mediated FasL gene. Methods The human lung adenocarcinoma cell line A-549 was transfected with the adenovirus-mediated FasL gene. The expression of caspase-3 and apoptotic morphological changes were measured using flow cytometry and...
AIM: To evaluate the role of survivin and caspase-3 in apoptosis of gastric carcinoma, as well as in prognosis of patients with gastric carcinoma. METHODS: Expressions of survivin and caspase-3 were investigated immunohistochemically in 80 gastric carcinoma patients without a history of chemo-radiation therapy. Tumor cell apoptosis was examined by TUNEL method. RESULTS: Immunohistochemical analysis...
Saikosaponin D is a saponin extract derived from several species of Bupleurum (Umbelliferae), which is used for the treatment of various liver diseases in traditional Chinese medicine. In this study, we report that Saikosaponin D inhibits the cell growth of human lung cancer cell line A549 and provide a molecular understanding of this effect. The results showed that Saikosaponin D inhibited the proliferation...
The objective of this study is to investigate the relationship between caspase-3 expression and apoptosis in the gastric adenocarcinoma cell line SGC-7901 transfected with the adenovirus-mediated FasL gene. The human gastric adenocarcinoma cell line SGC-7901 was transfected with the adenovirus-mediated FasL gene. The expression of caspase-3 and apoptotic morphological changes were measured by flow...
To investigate the apoptosis-inducing effect of Caspases-3 expressed by constructed eukaryotic vector on gastric cancer cell line SGC7901.PCR was employed to amplify the sequences of both small and large subunits of Caspases-3. Its products were separately cloned into the Sma I site of pBluescript KS+ to generate both plasmids pBS/SS and pBS/LS. The small subunit fragment was excised from plasmid...
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