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Various concentrations of kanamycin and paromomycin were examined for their effect on embryogenic cell suspension viability, transformation efficiency, and transgenic plant regeneration. Paromomycin (10–25mg/l) induced an earlier killing effect on cell suspensions than kanamycin (40–100mg/l). Transformation efficiency and the number of embryos developed on selection medium were positively correlated...
Embryogenic cell suspensions of grapevine (Vitis vinifera L.) were successfully cryopreserved by encapsulation-dehydration and subsequently regenerated into plants. Embryogenic cell suspensions were first-step precultured for 2 days in MGN liquid medium supplemented with increasing sucrose concentrations of 0.25, 0.5, 0.75 and 1 M. Precultured cells were then encapsulated and second-step precultured...
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