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A keratinolytic proteinase was cloned from thermophilic bacterium Geobacillus stearothermophilus AD-11 and was expressed in Escherichia coli BL21(DE3). Recombinant keratinolytic proteinase (RecGEOker) with an estimated molecular weight of 57kDa was purified and keratinase activity was measured. RecGEOker showed optimal activity at pH 9 and 60°C. Recombinant keratinolytic proteinase showed the highest...
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