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The Nicotiana tabacum × N. africana cross is a semilethal cross, where greater than 99 % of progeny die in the cotyledonary stage due to an interspecific lethality reaction. The low frequencies of surviving plants are mixtures of maternal tobacco haploids and aneuploid F1 hybrids. For effective use of surviving haploids in a breeding program, an efficient method is needed to distinguish them from...
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