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Differential Evolution is a competitive optimization technique over continuous space. The operation in the original Differential Evolution is simple, however, the mechanism, in the Differential Evolution makes it practically impossible to effectively use the original Differential Evolution to the binary space. A novel binary mutation operation was defined to enable the Differential Evolution to operate...
Trichoderma reesei CBS368, a strong endoglucanase 2 (EG 2) producing strain, was isolated in our laboratory. The DNA fragment encoding the EG 2 was cloned in pMD18-T and sequenced. In order to achieve its expression, the vector pET28a (+) and pET32a (+) were used as expression vector, and the recombinant EG 2 was successfully expressed in E. coli BL21 (DE3). Bioinformatic analysis was conducted using...
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