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A series of unsymmetric pentamethine cyanine dyes featuring benzoxazolyl and benzothiazolyl groups were synthesized, the structures were characterized by 1 H NMR, UV–vis absorption spectroscopy and mass spectrometry. The dyes with different anions such as chloride, tetraphenylborate and tetra(4-fluorophenyl)borate were also obtained and their solubility can be changed by the choice of the...
A series of fluorescein esters of straight chain fatty acid were synthesized and applied to the fluorometric assay of lipase. The rate of change in the fluorescence of the solution, due to the production of fluorescein, was measured and correlated with enzyme activity. The hydrolytic properties of fluorescein esters were investigated. In addition, the relationship between their hydrolytic properties...
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