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A rapid, high‐throughput, and quantitative method for cell entry route characterization is still lacking in nanomedicine research. Here, we report the application of imaging flow cytometry for quantitatively analyzing cell entry routes of actively targeted nanomedicines. We first engineered ICAM1 antibody‐directed fusogenic nanoliposomes (ICAM1‐FusoNLPs) and ICAM1 antibody‐directed endocytic nanolipogels...
The switch of tumor cells from a dormant, non‐angiogenic phenotype to an active, angiogenic phenotype is a critical step in early cancer progression. To date, relatively little is known about the cellular behaviors of angiogenic and non‐angiogenic tumor cell phenotypes. In this study, holographic imaging cytometry, a quantitative phase imaging (QPI) technique was used to continuously and non‐invasively...
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