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Light sheet microscopy allows rapid optical sectioning of whole chicken embryos during early development, visualizing cells through transgenic expression of a fluorescent cell membrane label. Low signal-to-noise ratios and large variability in intensity make segmentation of these images difficult, often resulting in discontinuous cell edges. We propose a multi-scale non-local means with shape prior...
The random walker method [1] has many nice characteristics for 3D image segmentation. However, it is computational expensive and slow due to a massive linear system to be solved. In this work, we take advantage of the probabilistic output from the random walker method applied to a small downsampled image. By using the original image and seeds information, a novel edge-preserving method is introduced...
We present a framework for interactive tracking of protein translocations between nuclei and cytoplasm of single cells. Initially, we segment selected keyframes using a novel interactive segmentation method, which employs a constrained density weighted Nyström method for eigenvector decomposition, and the geodesic commute distance for pixel classification. Tracking is achieved by both forward and...
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