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X-band electron paramagnetic resonance spectra of immobilized nitroxides were obtained by rapid scan at 293K. Scan widths were 155G with 13.4kHz scan frequency for 14N-perdeuterated tempone and for T4 lysozyme doubly spin labeled with an iodoacetamide spirocyclohexyl nitroxide and 100G with 20.9kHz scan frequency for 15N-perdeuterated tempone. These wide scans were made possible by modifications to...
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