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Listeria monocytogenes is a food‐borne pathogen that causes severe opportunistic infection in humans and animals. This study reports the development of single cross‐priming amplification (S‐CPA) and double CPA (D‐CPA) assays targeting species‐specific gene lmo0733 for identifying L. monocytogenes strains. The CPA assays were performed at a constant temperature 64 °C using seven specific primers and...
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