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A multi-color TIR-based device for multi-fluorescence imaging has been presented previously. Our proposed method integrated all miniaturized components, including cylindrical microlenses, prism, fiber alignment channels, into one monolithic PDMS chip, thus assembly is unnecessary, and misalignment is avoided. Here, we report new design and fabrication method of the device to improve the uniformity...
We report a novel, dual-color total internal reflection (TIR)-based chip which can generate two overlapping evanescent fields for simultaneous detection of two fluorophores. The chip was monolithically fabricated using Si bulk micromachining and PDMS casting. Our proposed method integrated all miniaturized components, including two cylindrical microlenses, one prism, two fiber alignment grooves and...
We report on a TIR-based chip which generates evanescent wave for ultra-sensitive fluorescent sensing. The chip is cast in PDMS using a Si mold fabricated by Si anisotropic wet etching and DRIE. Our method integrates miniaturized optical components, namely cylindrical microlens and prism into a monolithic chip; thus assembly is unnecessary, and misalignment is eliminated. The slide-format and monolithic...
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