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The regulation of nitrate reductase (NR, EC 1.6.6.3) in the mycorrhizal ascomycete Wilcoxina mikolae var. mikolae was studied with the aim of improving understanding of nitrate assimilation in mycorrhiza. It was possible to study induction, de-repression and repression of NR individually and in combination by utilizing the neutral nitrogen source urea. The NR activity required specific induction by...
Nitrate is an important source of nitrogen for ectomycorrhizal fungi and their hosts. Nitrate reductase catalyses the first step in the assimilation of nitrate into organic nitrogen and has not yet been successfully characterized from a mycorrhizal ascomycete. The extraction and properties of NR from the mycorrhizal Wilcoxina mikolae var. mikolae are described. The strategy adopted for the extraction...
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