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Three-dimensional (3-D) cell culture models have been developed to improve drug screening and predictive efficacy. In this study, a high-throughput drug screening system with autofluorescent cells cultured in 3-D polyethylene terephthalate (PET) scaffolds mimicking in vivo microenvironment was developed. Using a modified microbioreactor platform designed specifically for 3-D cell cultures, three commonly...
We have designed, built and tested a three-dimensional (3-D) cell culture system on modified microplates for high-throughput, real-time, proliferation and cytotoxicity assays. In this 3-D culture system, cells expressing the enhanced green fluorescent protein (EGFP) were cultured in nonwoven polyethylene terephthalate (PET) fibrous scaffolds. Compared to 2-D cultures in conventional microplates, 3-D...
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