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Aureobasidium pullulans is a yeast-like fungus used for producing biopolymers e.g. polymalic acid (PMA) and pullulan. A high PMA producing strain, A. pullulans CCTCC M2012223, was isolated and sequenced in our previous study. To understand its metabolic performance, a genome-scale metabolic model, iZX637, consisting of 637 genes, 1347 reactions and 1133 metabolites, was reconstructed based on genome...
Bio-based propionic acid production by propionibacteria is increasingly attractive because of its potential to replace petroleum-based chemical synthesis processes. Metabolic engineering of Propionibacterium freudenreichii subsp. shermanii DSM 4902 for enhanced propionic acid fermentation is made possible by the recently available whole genome sequence and genetic engineering tools. Pyruvate carboxylase...
Fumaric acid, a dicarboxylic acid used as a food acidulant and in manufacturing synthetic resins, can be produced from glucose in fermentation by Rhizopus oryzae. However, the fumaric acid yield is limited by the co-production of ethanol and other byproducts. To increase fumaric acid production, overexpressing endogenous pyruvate carboxylase (PYC) and exogenous phosphoenolpyruvate carboxylase (PEPC)...
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