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Iron (Fe) is an essential micronutrient for plants and citric acid acts as a chelate substance for the long-distance transmission of Fe. Citrate synthase (CS) protein is a key enzyme in the synthesis of citric acid. Using PCR amplification, a gene encoding a putative citrate synthase was isolated from Pyrus betulifolia, a widely-used rootstock native to China, and designated it as PbCS2. This gene...
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