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The DNA fragments of mpb63 and mpb64 were fused by splicing by overlapping extension(SOE) polymerase chain reaction(PCR), and the fusion gene mpb63–64 was cloned into pMD18-T vector, then we got the recombinant plasmid pMD-63–64. pMD-63–64 and pET28a(+) were digested by BamH□and EcoR□ double enzymes.The purified mpb63–64 fusion gene was subcloned into the expression vector pET28a(+), and the prokaryotic...
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