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Bacillus anthracis (B. anthracis), the causative agent of anthrax disease, is a Gram-positive spore-forming bacterium which can be used as a threatening bioterrorism agent. We developed enzyme-linked immunosorbent assay (ELISA)-on-a-chip biosensors for rapid, sensitive analysis of B. anthracis spores based on two-dimensional, cross-flow chromatography. In order to establish optimal assay conditions,...
A chemiluminometric biosensor system for point-of-care testing has been developed using an immuno-chromatographic assay combined with an enzyme (e.g., horseradish peroxidase) tracer that produces a light signal measurable on a simple detector. Cross-flow chromatography, a method previously investigated by our laboratory, was utilized in order to accomplish sequential antigen–antibody binding and signal...
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