Search results for: Joe Tohme

Items from 1 to 20 out of 36 results

article

Translocation of a parthenogenesis gene candidate to an alternate carrier chromosome in apomictic Brachiaria humidicola

Margaret Worthington, Masumi Ebina, Naoki Yamanaka, Christopher Heffelfinger, more

BMC Genomics > 2019 > 20 > 1 > 1-18

Background The apomictic reproductive mode of Brachiaria (syn. Urochloa) forage species allows breeders to faithfully propagate heterozygous genotypes through seed over multiple generations. In Brachiaria, reproductive mode segregates as single dominant locus, the apospory-specific genomic region (ASGR). The AGSR has been mapped to an area of reduced recombination on Brachiaria decumbens chromosome...

article

Molecular analysis of the expression of a crtB transgene and the endogenous psy2-y₁ and psy2-y₂ genes of cassava and their effect on root carotenoid content

Paul Chavarriaga-Aguirre, Mónica Prías, Danilo López, Darwin Ortiz, more

Transgenic Research > 2017 > 26 > 5 > 639-651

A conventional breeding program was established to transfer the bacterial phytoene synthase transgene–crtB–from a transgenic, white-rooted cassava to yellow-rooted cassava plants carrying the endogenous phytoene synthase alleles named psy2-y₁ and/or psy2-y₂. Combining endogenous phytoene synthase enzymes (PSYs) with CRTB in a single cassava plant would allow the molecular dissection of individual...

article

The potential of using biotechnology to improve cassava: a review

Paul Chavarriaga-Aguirre, Alejandro Brand, Adriana Medina, Mónica Prías, more

In Vitro Cellular & Developmental Biology - Plant > 2016 > 52 > 5 > 461-478

The importance of cassava as the fourth largest source of calories in the world requires that contributions of biotechnology to improving this crop, advances and current challenges, be periodically reviewed. Plant biotechnology offers a wide range of opportunities that can help cassava become a better crop for a constantly changing world. We therefore review the state of knowledge on the current use...

article

A global alliance declaring war on cassava viruses in Africa

James Legg, Eklou Attiogbevi Somado, Ian Barker, Larry Beach, more

Food Security > 2014 > 6 > 2 > 231-248

article

In‐depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches

Mathias Lorieux, Mélisande Blein, Jaime Lozano, Mathieu Bouniol, more

Plant Biotechnology Journal > 10 > 5 > 555 - 568

We report here the molecular and phenotypic features of a library of 31 562 insertion lines generated in the model japonica cultivar Nipponbare of rice (Oryza sativa L.), called Oryza Tag Line (OTL). Sixteen thousand eight hundred and fourteen T‐DNA and 12 410 Tos17 discrete insertion sites have been characterized in these lines. We estimate that 8686 predicted gene intervals—i.e. one‐fourth to one‐fifth...

article

Evaluating purple passion fruit (Passiflora edulis Sims f. edulis) genetic variability in individuals from commercial plantations in Colombia

Diana Carolina Ortiz, Adriana Bohórquez, Myriam Cristina Duque, Joe Tohme, more

Genetic Resources and Crop Evolution > 2012 > 59 > 6 > 1089-1099

This study evaluated P. edulis f. edulis intraspecific variation at DNA level using AFLPs and SSRs. About 60 specimens were collected from Colombian commercial plantations in the departments of Antioquia, Boyacá, Cundinamarca, Huila, Quindío and Tolima. Ten specimens were also included from Corpoica’s La Selva research center Passiflora germplasm bank. A non-commercial purple passion fruit plant and...

article

Identification and Characterization of the Cassava Core-Clock Gene EARLY FLOWERING 4

Oluwabusayo Sarah Adeyemo, Elsebeth Kolmos, Joe Tohme, Paul Chavariaga, more

Tropical Plant Biology > 2011 > 4 > 2 > 117-125

The angiosperm circadian clock has been well established from molecular-genetic studies in a temperate plant model. Conservation of clock function is less explored in plants from the tropics. Cassava (Manihot esculenta) is a staple crop grown in the tropics that has been of limited research interest, and more generally, research on photoperiod and clock genes has been sparse. EARLY FLOWERING 4 (AtELF4...

article

Genomic comparison between Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola, using suppression‐subtractive hybridization

Mauricio Soto‐Suárez, Carolina González, Benoît Piégu, Joe Tohme, more

FEMS Microbiology Letters > 308 > 1 > 16 - 23

Xanthomonas oryzae pathovar oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc) cause bacterial diseases in rice: leaf blight and leaf streak, respectively. Although both the Asian and the African strains of Xoo induce similar symptoms, they are genetically different, with the African Xoo strains being more closely related to the Asian Xoc. To identify the sequences responsible for differences between...

article

A universal core genetic map for rice

Julie Orjuela, Andrea Garavito, Matthieu Bouniol, Juan David Arbelaez, more

Theoretical and Applied Genetics > 2010 > 120 > 3 > 563-572

To facilitate the creation of easily comparable, low-resolution genetic maps with evenly distributed markers in rice (Oryza sativa L.), we conceived of and developed a Universal Core Genetic Map (UCGM). With this aim, we derived a set of 165 anchors, representing clusters of three microsatellite or simple sequence repeat (SSR) markers arranged into non-recombining groups. Each anchor consists of at...

article

Phylogenetic patterns in the genus Manihot (Euphorbiaceae) inferred from analyses of nuclear and chloroplast DNA regions

Juliana Chacón, Santiago Madriñán, Daniel Debouck, Fausto Rodriguez, more

Molecular Phylogenetics and Evolution > 2008 > 49 > 1 > 260-267

From a phylogenetic perspective, the genus Manihot can be considered as an orphan group of plants, and the scientific knowledge acquired has been mainly related to cassava, one of the most important crops in poor tropical countries. The goal of the majority of evolutionary studies in the genus has been to decipher the domestication process and identify the closest relatives of cassava. Few investigations...

article

BAC-end Sequence Analysis and a Draft Physical Map of the Common Bean (Phaseolus vulgaris L.) Genome

Jessica A. Schlueter, Jose Luis Goicoechea, Kristi Collura, Navdeep Gill, more

Tropical Plant Biology > 2008 > 1 > 1 > 40-48

Common bean (Phaseolus vulgaris L.) is a legume that is an important source of dietary protein in developing countries throughout the world. Utilizing the G19833 BAC library for P. vulgaris from Clemson University, 89,017 BAC-end sequences were generated giving 62,588,675 base pairs of genomic sequence covering approximately 9.54% of the genome. Analysis of these sequences in combination with 1,404...

article

Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice

Jorge Luis Fuentes, Fernando José Correa-Victoria, Fabio Escobar, Gustavo Prado, more

Euphytica > 2008 > 160 > 3 > 295-304

The present work was conducted to identify microsatellite markers linked to the rice blast resistance gene Pi-1(t) for a marker-assisted selection program. Twenty-four primer pairs corresponding to 19 microsatellite loci were selected from the Gramene database (www. gramene.org) considering their relative proximity to Pi-1(t) gene in the current rice genetic map. Progenitors and DNA bulks of resistant...

article

Towards identifying the full set of genes expressed during cassava post-harvest physiological deterioration

Kim Reilly, Diana Bernal, Diego F. Cortés, Rocío Gómez-Vásquez, more

Plant Molecular Biology > 2007 > 64 > 1-2 > 187-203

Storage roots of cassava (Manihot esculenta Crantz) exhibit a rapid post-harvest physiological deterioration (PPD) response that can occur within 24–72 h of harvest. PPD is an enzymatically mediated oxidative process with parallels to plant wound, senescence and defence responses. To characterise those genes that show significant change in expression during the PPD response we have used cDNA microarray...

article

Through the genetic bottleneck: O. rufipogon as a source of trait-enhancing alleles for O. sativa

Susan R. McCouch, Megan Sweeney, Jiming Li, Hui Jiang, more

Euphytica > 2007 > 154 > 3 > 317-339

This paper summarizes results from a decade of collaborative research using advanced backcross (AB) populations to a) identify quantitative trait loci (QTL) associated with improved performance in rice and to b) clone genes underlying key QTLs of interest. We demonstrate that AB-QTL analysis is capable of (1) successfully uncovering positive alleles in wild germplasm that were not obvious based on...

article

Gene expression profile in response to Xanthomonas axonopodis pv. manihotis infection in cassava using a cDNA microarray

Camilo Lopez, Mauricio Soto, Silvia Restrepo, Benoît Piégu, more

Plant Molecular Biology > 2005 > 57 > 3 > 393-410

A cassava cDNA microarray based on a large cassava EST database was constructed and used to study the incompatible interaction between cassava and Xanthomonas axonopodis pv. manihotis (Xam) strain CIO151. For microarray construction, 5700 clones from the cassava unigene set were amplified by polymerase chain reaction (PCR) and printed on glass slides. Microarray hybridization was performed using cDNA...

article

Integration of genomics approach with traditional breeding towards improving abiotic stress adaptation: drought and aluminum toxicity as case studies

Manabu Ishitani, Idupulapati Rao, Peter Wenzl, Steve Beebe, more

Field Crops Research > 2004 > 90 > 1 > 35-45

Traditional breeding efforts are expected to be greatly enhanced through collaborative approaches incorporating functional, comparative and structural genomics. Potential benefits of combining genomic tools with traditional breeding have been a source of widespread interest and resulted in numerous efforts to achieve the desired synergy among disciplines. The International Center for Tropical Agriculture...

article

Genetic diversity of traditional South American Landraces of Cassava (Manihot esculenta Crantz): an analysis using microsatellites

Marianne Elias, Gilda Santos Mühlen, Doyle McKey, Ana Carolina Roa, more

Economic Botany > 2004 > 58 > 2 > 242-256

The extent and structure of the genetic variability of traditional varieties of cassava (Manihot esculenta Crantz) have been little documented, despite considerable evidence for this cropś great varietal diversity in traditional agroecosystems. We used microsatellite markers to assess the genetic structure of traditional landraces of sweet and bitter cassava collected from five South American sites...

article

The Global Cassava Partnership for Genetic Improvement

Claude M. Fauquet, Joe Tohme

Plant Molecular Biology > 2004 > 56 > 4 > 5-10

article

A unigene catalogue of 5700 expressed genes in cassava

Camilo Lopez, Véronique Jorge, Benoît Piégu, Chickelu Mba, more

Plant Molecular Biology > 2004 > 56 > 4 > 541-554

Two economically important characters, starch content and cassava bacterial blight resistance, were targeted to generate a large collection of cassava ESTs. Two libraries were constructed from cassava root tissues of varieties with high and low starch contents. Other libraries were constructed from plant tissues challenged by the pathogen Xanthomonas axonopodis pv.manihotis. We report here the single...

article

An EST resource for cassava and other species of Euphorbiaceae

James V. Anderson, Michel Delseny, Martin A. Fregene, Veronique Jorge, more

Plant Molecular Biology > 2004 > 56 > 4 > 527-539

Cassava (Manihot esculenta) is a major food staple for nearly 600 million people in Africa, Asia, and Latin America. Major losses in yield result from biotic and abiotic stresses that include diseases such as Cassava Mosaic Disease (CMD) and Cassava Bacterial Blight (CBB), drought, and acid soils. Additional losses also occur from deterioration during the post-harvest storage of roots. To help cassava...

Publication date

Set your own date range

INFONA - science communication portal

Search results for: Joe Tohme

Translocation of a parthenogenesis gene candidate to an alternate carrier chromosome in apomictic Brachiaria humidicola

Molecular analysis of the expression of a crtB transgene and the endogenous psy2-y₁ and psy2-y₂ genes of cassava and their effect on root carotenoid content

The potential of using biotechnology to improve cassava: a review

A global alliance declaring war on cassava viruses in Africa

In‐depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches

Evaluating purple passion fruit (Passiflora edulis Sims f. edulis) genetic variability in individuals from commercial plantations in Colombia

Identification and Characterization of the Cassava Core-Clock Gene EARLY FLOWERING 4

Genomic comparison between Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola, using suppression‐subtractive hybridization

A universal core genetic map for rice

Phylogenetic patterns in the genus Manihot (Euphorbiaceae) inferred from analyses of nuclear and chloroplast DNA regions

BAC-end Sequence Analysis and a Draft Physical Map of the Common Bean (Phaseolus vulgaris L.) Genome

Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice

Towards identifying the full set of genes expressed during cassava post-harvest physiological deterioration

Through the genetic bottleneck: O. rufipogon as a source of trait-enhancing alleles for O. sativa

Gene expression profile in response to Xanthomonas axonopodis pv. manihotis infection in cassava using a cDNA microarray

Integration of genomics approach with traditional breeding towards improving abiotic stress adaptation: drought and aluminum toxicity as case studies

Genetic diversity of traditional South American Landraces of Cassava (Manihot esculenta Crantz): an analysis using microsatellites

The Global Cassava Partnership for Genetic Improvement

A unigene catalogue of 5700 expressed genes in cassava

An EST resource for cassava and other species of Euphorbiaceae

Filter options

Publication date

Keywords

Data set

Journal

INFONA - science communication portal

Search results for: Joe Tohme

Add recipient

Sending message cancelled

Are you sure you want to cancel sending this message?

Send message

Filter options

Publication date

Date range setting

Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.

Keywords

Data set

Journal

Reporting an error / abuse

Sending the report failed

Accessibility options