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This paper proposes a formation method for oil-layer-free bilayer lipid membranes (BLM) by using a compressive force of DC electric field. Using a droplet contact device previously developed, constant-voltage steps were applied to the pre-bilayer membrane and carefully controlled the electric stress in response to the membrane features, based on optical and current monitoring. We examined the pathways...
This paper describes an investigation of drug effects for ion channels with recognizing the intra/extracellular directions. We have previously reported the ion channel recordings with bilayer lipid membranes (BLMs) which formed on a parylene micropore using droplets contact method. However, the low reconstitution probability of ion channels into BLMs has been a major issue. In this study, BLMs area...
This paper describes a method to form bilayer lipid membranes by splitting, sliding and contacting water droplets in oil containing lipid. A droplet surrounded by a lipid monolayer, which is easily formed by injecting an aqueous droplet into an organic solvent that contains lipids, is split and then, brought into contact each other. A bilayer lipid membrane is immediately formed at the interface of...
Transmembrane proteins are important in biological functions. Proteoliposomes, which contain reconstituted membrane proteins, have been considered to be useful for their investigation. Especially, cell-sized giant liposomes, or giant unilamellar vesicles, are often used for such purposes. Previously, we established a novel method of proteoliposome preparation using membrane fusion between recombinant...
Giant liposomes are cell-size artificial vesicles with phospholipid bilayers. Here, in order to obtain giant liposomes containing membrane proteins (giant proteoliposomes), we have introduced a novel procedure using membrane fusion phenomena between liposomes and recombinant baculovirus. We tried to thereby reconstitute heterotrimeric guanine nucleotide-binding protein (G protein) that is a peripheral...
Giant unilamellar vesicles (GUVs), or giant liposomes (GLs), are biochemical compartments enclosed with phospholipid bilayers. The size of GUVs is comparable to that of real cells (~10-100 mum). Thus, GUVs are employed to construct artificial cell models, which are reconstituted from biochemical materials to mimic real cellular functions in a simpler manner for investigation of fundamental mechanisms...
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