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We explore whether antisense oligodeoxy-nucleotides (ASODN) targeting survivin gene can reverse apoptotic resistance induced by cisplatin in human lung adenocarcinoma cells. A549 / CDDP cell lines were cultured routinely with RPMI-1640 medium. Survivin ASODN mediated by cytofectin was transfected into the A549 / CDDP cells. The influence of ASODN transfection on apoptosis was determined by Diphenylamine...
This study was designed to assess E-cadherin (E-cad)and proliferating cell nuclear antigen (PCNA)expression as well as their clinicopathological significance in human non small cell lung cancers (NSCLCs). Possible molecular mechanisms of differentiation and metastasis of NSCLCs are discussed. Immuno-histochemical and immunofluorescence double staining were performed to examine the expression of E-cad...
The aim of this study was to investigate the expression of Fas and Fas ligand (FasL) and to determine the signifficance of these molecules in lung cancer cell lines. Immunoblotting, RT- PCR and flow cytometric analyses were carried out to measure the expression of Fas and FasL and to examine their interactions and e.ects on cell growth and apoptosis. Fas and FasL were co-expressed in most of the cell...
Lack of surface Fas expression is a main route for apoptotic resistance which is considered an important mechanism of tumorigenesis and tumor progression. Fas and FasL expressions in 110 non-small cell lung carcinomas (NSCLCs) were investigated to evaluate their roles in pulmonary carcinogenesis and to examine the clinicopathologic significance of Fas expression with its relationship with p53 and...
Objective: To study the relationship between the methylation status of multi-drug resistance protein (MRP) gene and the expression of its mRNA and protein in lung cancer cell lines. Methods: Human lung cell line WI-38.1ung adenocarcinoma cell line SPCA-1 and its drug-resistant cells induced by different concentrations of doxorubicin were treated with restriction endonuclease Eco47III. The methylation...
AIM: Lack of surface Fas expression is a main route for apoptotic resistance which is considered an important mechanism of tumorigenesis and tumor progression. Methods: Fas and FasL expressions in 110 non-small cell lung carcinomas (NSCLCs) were investigated to evaluate their roles in pulmonary carcinogenesis and to examine the clinicopathologic significance of Fas expression with its relationship...
To observe the influence of hyperthermia under different temperature on the growth, apoptosis of Human Lung Cancer cell of A549, H1299 to investigate the possible mechanism of apoptosis in lung cancer cell caused by p53 with different activity. Human lung cancer cell of A549, H1299 was heated under 40??C 42??C 44??C separately for 2 hours each in the water bath case. After the hyperthermia under different...
Objective: To evaluate the clinical significance of the aberrant methylation of DAPK gene and p16 gene in serum from 65 NSCLC patients from the first affiliated hospital of suzhou university. Methods : A methylation-specific PCR (MSP)was performed for the detection of promoter hypermethylation of DAPK gene and p16 gene in blood DNA from 65 cases of NSCLC. and to analyze the relation of the aberrant...
With the advance of immunology, immunogene therapy is becoming a possible therapeutic alternative to advanced cancer. The aim of tumor immunogene therapy is to enhance the immune response to tumors. Evidence suggests that CD80 (B7-1) and Interferon-gamma (IFN-gamma) play important roles in antitumor immunity induced by T lymphocyte. To study the antitumor immune effects of lung carcinoma vaccine modified...
AIM: In the present study, murine A549 Lung cancer cells were provided Lung cancer with different thermal dose invitro and invivo, thereafter we investigated the apoptosis, necrosis rates, and intratumoral microvessel density (MVD) to determine that microvessel damage plays an important role in the tumoricidal effect of Lung cancer. Methods: A549 Lung cancer cells were inoculated in the right hind...
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