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We investigate the anticancer effect of a traditional Chinese medicine gambogic acid (GA) in human gastric cancer line SGC-7901 and further study the mechanism of apoptosis induction of GA.Low differential human gastric cancer line SGC-7901 were treated with GA at different doses and different times, the inhibitory rates were detected by MTT assay. Apoptosis induced by GA in SGC-7901 cells was observed...
We explore whether antisense oligodeoxy-nucleotides (ASODN) targeting survivin gene can reverse apoptotic resistance induced by cisplatin in human lung adenocarcinoma cells. A549 / CDDP cell lines were cultured routinely with RPMI-1640 medium. Survivin ASODN mediated by cytofectin was transfected into the A549 / CDDP cells. The influence of ASODN transfection on apoptosis was determined by Diphenylamine...
We investigate the effects of anti-Fas ribozyme on Fas expression and apoptosis in primary cultured Neonatal mouse ventricular myocytes. Neonatal mouse ventricular myocytes were isolated by using collagenase irrigation. A hammerhead ribozyme targeting the Fas mRNA was constructed, and transfected into Neonatal mouse ventricular myocytes via Effectene. Then Fas expression in Neonatal mouse ventricular...
Objective: To study the relationship between the methylation status of multi-drug resistance protein (MRP) gene and the expression of its mRNA and protein in lung cancer cell lines. Methods: Human lung cell line WI-38.1ung adenocarcinoma cell line SPCA-1 and its drug-resistant cells induced by different concentrations of doxorubicin were treated with restriction endonuclease Eco47III. The methylation...
Objective: To evaluate the clinical significance of the aberrant methylation of DAPK gene and p16 gene in serum from 65 NSCLC patients from the first affiliated hospital of suzhou university. Methods : A methylation-specific PCR (MSP)was performed for the detection of promoter hypermethylation of DAPK gene and p16 gene in blood DNA from 65 cases of NSCLC. and to analyze the relation of the aberrant...
AIM: In the present study, murine A549 Lung cancer cells were provided Lung cancer with different thermal dose invitro and invivo, thereafter we investigated the apoptosis, necrosis rates, and intratumoral microvessel density (MVD) to determine that microvessel damage plays an important role in the tumoricidal effect of Lung cancer. Methods: A549 Lung cancer cells were inoculated in the right hind...
Evaluate the possible value of FasL in gastric cancer gene therapy by investigating the effects of FasL expression on human gastric cancer cell line.An adenoviral vector encoding the full-length human FasL cDNA was constructed and used to infect a human gastric cancer (SGC-7901) cell line. FasL expression was confirmed by X-gal staining, flow cytometric analysis and RT-PCR. The effect of FasL on cell...
To investigate the apoptosis-inducing effect of Caspases-3 expressed by constructed eukaryotic vector on gastric cancer cell line SGC7901.PCR was employed to amplify the sequences of both small and large subunits of Caspases-3. Its products were separately cloned into the Sma I site of pBluescript KS+ to generate both plasmids pBS/SS and pBS/LS. The small subunit fragment was excised from plasmid...
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