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Glucagon-like peptide-1 (7–36) amide (GLP-1), a gut hormone released into the blood stream after feeding, can stimulate insulin secretion by potentiating the insulinotropic action of glucose. An expression vector pET-22bG8, encoding a fusion protein containing eight tandem repeat GLP-1 ([Ser8, Gln26, Asp34]-GLP-1) analogues, was constructed and transformed into the Escherichia coli BL21(DE3) strain...
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