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We report the construction of an ∼1.7-Mb sequence-ready YAC/BAC clone contig of 8p22–p23. This chromosomal region has been associated with frequent loss of heterozygosity (LOH) in breast, ovarian, prostate, head and neck, and liver cancer. We first constructed a meiotic linkage map for 8p to resolve previously reported conflicting map orders from the literature. The target region containing a putative...
Nearest neighbor analysis (NNA) adjusts for spatially correlated residuals, with the goal of increasing precision. The magnitude of the block treatment interaction mean square is commonly used to evaluate the precision of the NNA model. An alternative method of evaluating the precision of the NNA and classical unadjusted (UNADJ) randomized complete block (RCB) analysis would be to use the pooled variance...
A YAC library enriched for telomere clones was constructed and screened for the human telomere-specific repeat sequence (TTAGGG). Altogether 196 TYAC library clones were studied: 189 new TYAC clones were isolated, 149 STSs were developed for 132 different TYACs, and 39 P1 clones were identified using 19 STSs from 16 of the TYACs. A combination of mapping methods including fluorescencein situhybridization,...
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