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Ascoviruses are double‐stranded DNA viruses that are pathogenic to noctuid larvae. In vitro infection causes the cells to fail to replicate and proliferate normally. However, the molecular mechanisms are unclear. In this study, the transmission electron microscopy data of infected‐Spodoptera exigua (Hübner) fat body cells (SeFB, IOZCAS‐SpexII‐A cells) showed that virions were internalized in phagocytic...