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Background CYP106A2 from Bacillus megaterium ATCC 13368 was first identified as a regio- and stereoselective 15β-hydroxylase of 3-oxo-∆ 4 -steroids. Recently, it was shown that besides 3-oxo-∆ 4 -steroids, 3-hydroxy-∆ 5 -steroids as well as di- and triterpenes can also serve as substrates for this biocatalyst. It is highly selective towards...
CYP105A1 from Streptomyces griseolus belongs to a widespread family of soluble prokaryotic cytochromes P450. For in vitro studies we established an electron transfer system, consisting of the ferredoxin Etp1 fd and the ferredoxin reductase Arh1 from the fission yeast Schizosaccharomyces pombe. We investigated the metabolism of glibenclamide and glimepiride, hypoglycemic drugs of sulfonylurea...
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