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Successful application of doubled-haploid (DH) technology in onion (Allium cepa L.) breeding programs requires production of large numbers of fertile DH plants. Only a small percentage of the onion plants recovered via gynogenesis are spontaneous DH, so induction of chromosome doubling is required. The chromosome doubling step is currently one of the major limitations in onion DH programs. To address...
We report the successful production of large numbers of fecund spontaneous and induced doubled haploid (DH) onion lines from pungent and mild materials developed in our long day onion breeding program. Culture of 3-5mm unopened flower buds on modified BDS medium was the most efficient and convenient method for recovery of gynogenic plants. Over 1100 gynogenic plants were produced from approximately...
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