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The spray gene was cloned, and wildtype and mutant alleles were sequenced. Spray+ has a 3452-bp open reading frame plus seven introns. The spray mutant had a T → G transversion close to the carboxyl end, creating a stop codon (TGA). The sequence shows no match to genes of known function, but the carboxyl end shows seven transmembrane domains and matches putative membrane proteins of yeast. The most...
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