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Our aim in this study is to show that IgY antibody based immunoassays could be used to detect antibiotic residues in animal-derived food. Briefly, full antigens of gentamicin (Gent) and kanamycin (Kana) were used to immunize the laying chickens to prepare IgY antibodies. Then, these antibodies were evaluated by FPIA and ic-ELISA to detect Gent/Kana in animal-derived samples. The IC50 of FPIA and ic-ELISA...
Zearalenone (ZEN) is one of the most common contaminants in fodder with obvious reproduction toxicity and potential carcinogenicity to animals and humans. Thus, simple and sensitive methods are required for the detection of ZEN. In this work, the anti-ZEN monoclonal antibody (mAb) was prepared by hybridoma technique. Then, the mAb-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA),...
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