Search results for: Garry Wong

Items from 1 to 20 out of 42 results

article

Identification of potential blood biomarkers for Parkinson’s disease by gene expression and DNA methylation data integration analysis

Changliang Wang, Liang Chen, Yang Yang, Menglei Zhang, more

Clinical Epigenetics > 2019 > 11 > 1 > 1-15

Background Blood-based gene expression or epigenetic biomarkers of Parkinson’s disease (PD) are highly desirable. However, accuracy and specificity need to be improved, and methods for the integration of gene expression with epigenetic data need to be developed in order to make this feasible. Methods Whole blood gene expression data and DNA methylation data were downloaded from Gene Expression Omnibus...

article

Human amyloid β peptide and tau co-expression impairs behavior and causes specific gene expression changes in Caenorhabditis elegans

Chenyin Wang, Valeria Saar, Ka Lai Leung, Liang Chen, more

Neurobiology of Disease > 2018 > 109 > PA > 88-101

Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by the presence of extracellular amyloid plaques consisting of Amyloid-β peptide (Aβ) aggregates and neurofibrillary tangles formed by aggregation of hyperphosphorylated microtubule-associated protein tau. We generated a novel invertebrate model of AD by crossing Aβ1–42 (strain CL2355) with either pro-aggregating tau...

chapter

Novel tumor biomarker based on isomiR expression profiles

Liang Chen, Garry Wong

2017 IEEE International Conference on Bioinformatics and Biomedicine (BIBM) > 2328 - 2329

2017 IEEE International Conference on Bioinformatics and Biomedicine (BIBM)

With the development of deep sequencing technology, isomiRs (isoform of miRNA) are consistently observed in a variety of cell types, tissues, and different cell development stages. miRNA isoforms as the products of miRNA genes, are variants which are different from mature miRNAs in length and position. Recently, many studies emphasized on isomiR and found its subtypes are differentially expression...

article

Chronic MeHg exposure modifies the histone H3K4me3 epigenetic landscape in Caenorhabditis elegans

Martina Rudgalvyte, Juhani Peltonen, Merja Lakso, Garry Wong

Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology > 2017 > 191 > C > 109-116

Methylmercury (MeHg) is a persistent environmental pollutant that occurs in the food chain, at occupational sites, and via medical procedures. Exposure in humans and animal models results in renal, neuro, and reproductive toxicities. In this study, we demonstrate that chronic exposure to MeHg (10μM) causes epigenetic landscape modifications of histone H3K4 trimethylation (H3K4me3) marks in Caenorhabditis...

article

RNA‐Seq Reveals Acute Manganese Exposure Increases Endoplasmic Reticulum Related and Lipocalin mRNAs in Caenorhabditis elegans

Martina Rudgalvyte, Juhani Peltonen, Merja Lakso, Richard Nass, more

Journal of Biochemical and Molecular Toxicology > 30 > 2 > 97 - 105

Manganese (Mn) is an essential nutrient; nonetheless, excessive amounts can accumulate in brain tissues causing manganism, a severe neurological condition. Previous studies have suggested oxidative stress, mitochondria dysfunction, and impaired metabolism pathways as routes for Mn toxicity. Here, we used the nematode Caenorhabditis elegans to analyze gene expression changes after acute Mn exposure...

article

Evolutionary conservation and function of the human embryonic stem cell specific miR-302/367 cluster

Liang Chen, Liisa Heikkinen, K. Emily Knott, Yanchun Liang, more

Comparative Biochemistry and Physiology Part D: Genomics and Proteomics > 2015 > 16 > C > 83-98

miRNA clusters define a group of related miRNAs closely localized in the genome with an evolution that remains poorly understood. The miR-302/367 cluster represents a single polycistronic transcript that produces five precursor miRNAs. The cluster is highly expressed and essential for maintenance of human embryonic stem cells. We found the cluster to be highly conserved and present in most mammals...

article

Transcriptional profiling reveals differential expression of a neuropeptide-like protein and pseudogenes in aryl hydrocarbon receptor-1 mutant Caenorhabditis elegans

Vuokko Aarnio, Liisa Heikkinen, Juhani Peltonen, Gundars Goldsteins, more

Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics > 2014 > 9 > Complete > 40-48

The aryl hydrocarbon receptor (AHR) functions in higher organisms in development, metabolism and toxic responses. Its Caenorhabditis elegans (C. elegans) ortholog, AHR-1, facilitates neuronal development, growth and movement. We investigated the effect of AHR mutation on the transcriptional profile of L4 stage C. elegans using RNA-seq and quantitative real time PCR in order to understand better AHR-1...

article

Methylmercury exposure increases lipocalin related (lpr) and decreases activated in blocked unfolded protein response (abu) genes and specific miRNAs in Caenorhabditis elegans

Martina Rudgalvyte, Natalia VanDuyn, Vuokko Aarnio, Liisa Heikkinen, more

Toxicology Letters > 2013 > 222 > 2 > 189-196

Methylmercury (MeHg) is a persistent environmental and dietary contaminant that causes serious adverse developmental and physiologic effects at multiple cellular levels. In order to understand more fully the consequences of MeHg exposure at the molecular level, we profiled gene and miRNA transcripts from the model organism Caenorhabditis elegans. Animals were exposed to MeHg (10μM) from embryo to...

article

Chronic Ethanol Exposure Increases Cytochrome P‐450 and Decreases Activated in Blocked Unfolded Protein Response Gene Family Transcripts in Caenorhabditis elegans

Juhani Peltonen, Vuokko Aarnio, Liisa Heikkinen, Merja Lakso, more

Journal of Biochemical and Molecular Toxicology > 27 > 3 > 219 - 228

Ethanol is a widely consumed and rapidly absorbed toxin. While the physiological effects of ethanol consumption are well known, the underlying biochemical and molecular changes at the gene expression level in whole animals remain obscure. We exposed the model organism Caenorhabditis elegans to 0.2 M ethanol from the embryo to L4 larva stage and assayed gene expression changes in whole animals using...

article

Radiation-induced genomic instability in Caenorhabditis elegans

Katriina Huumonen, Hanna-Kaisa Immonen, Keith Baverstock, Mikko Hiltunen, more

Mutation Research - Genetic Toxicology and Environmental Mutagenesis > 2012 > 748 > 1-2 > 36-41

Radiation-induced genomic instability has been well documented, particularly in vitro. However, the understanding of its mechanisms and their consequences in vivo is still limited. In this study, Caenorhabditis elegans (C. elegans; strain CB665) nematodes were exposed to X-rays at doses of 0.1, 1, 3 or 10Gy. The endpoints were measured several generations after exposure and included mutations in the...

article

Trans fat diet causes decreased brood size and shortened lifespan in Caenorhabditis elegans delta‐6‐desaturase mutant fat‐3

Kaja Reisner, Marko Lehtonen, Markus Storvik, Tanel Jantson, more

Journal of Biochemical and Molecular Toxicology > 25 > 5 > 269 - 279

Trans‐fatty acids (TFAs) enter the diet through industrial processes and can cause adverse human health effects. The present study was aimed to examine the effects of dietary cis‐ and trans‐fatty acids on the model organism Caenorhabditis elegans. Cis‐ or trans‐18:1n9 triglycerides (25 μM) caused no apparent changes in the numbers of viable progeny of wild‐type N2 animals. However, in fat‐3 mutants...

article

Determination of endocannabinoids in nematodes and human brain tissue by liquid chromatography electrospray ionization tandem mass spectrometry

Marko Lehtonen, Markus Storvik, Hanna Malinen, Petri Hyytiä, more

Journal of Chromatography B > 2011 > 879 > 11-12 > 677-694

A simple and highly sensitive liquid chromatography/tandem mass spectrometric (LC/MS/MS) method was developed to compare endogenous cannabinoid levels in nematodes and in brains of rats and humans, with and without prior exposure to ethanol. After liquid–liquid extraction of the lipid fraction from homogenized samples, a reversed-phase sub 2μm column was used for separating analytes with an isocratic...

article

Overexpression of SUMO perturbs the growth and development of Caenorhabditis elegans

Miia M. Rytinki, Merja Lakso, Petri Pehkonen, Vuokko Aarnio, more

Cellular and Molecular Life Sciences > 2011 > 68 > 19 > 3219-3232

Small ubiquitin-related modifiers (SUMOs) are important regulator proteins. Caenorhabditis elegans contains a single SUMO ortholog, SMO-1, necessary for the reproduction of C. elegans. In this study, we constructed transgenic C. elegans strains expressing human SUMO-1 under the control of pan-neuronal (aex-3) or pan-muscular (myo-4) promoter and SUMO-2 under the control of myo-4 promoter. Interestingly,...

article

Genes regulated in MPTP-treated macaques and human Parkinson's disease suggest a common signature in prefrontal cortex

Markus Storvik, Marie-Jeanne Arguel, Sandra Schmieder, Audrey Delerue-Audegond, more

Neurobiology of Disease > 2010 > 38 > 3 > 386-394

The presymptomatic phase of Parkinson's disease (PD) is now recognized as a prodromal phase, with compensatory mechanism masking its progression and non-motor early manifestations, such as depression, cognitive disturbances and apathy. Those mechanisms were thought to be strictly dopamine-mediated until recent advances have shed light upon involvement of putative outside-basal ganglia, i.e. cortical,...

article

Fatty acid composition and gene expression profiles are altered in aryl hydrocarbon receptor-1 mutant Caenorhabditis elegans

Vuokko Aarnio, Markus Storvik, Marko Lehtonen, Suvi Asikainen, more

Comparative Biochemistry and Physiology, Part C > 2010 > 151 > 3 > 318-324

The aryl hydrocarbon receptor (AHR) is a eukaryotic transcription factor that plays an essential role in neuronal, immune, vascular, hepatic and hematopoietic development. In mammals, AHR induces metabolism-associated genes in response to xenobiotics. AHR is evolutionarily conserved, and the C. elegans AHR ortholog likely shares many physiologic functions with the mammalian version. While the role...

article

Global microRNA Expression Profiling of Caenorhabditis elegans Parkinson's Disease Models

Suvi Asikainen, Martina Rudgalvyte, Liisa Heikkinen, Kristiina Louhiranta, more

Journal of Molecular Neuroscience > 2010 > 41 > 1 > 210-218

MicroRNAs (miRNAs) play an important role in human brain development and maintenance. To search for miRNAs that may be involved in the pathogenesis of Parkinsons disease (PD), we utilized miRNA microarrays to identify potential gene expression changes in 115 annotated miRNAs in PD-associated Caenorhabditis elegans models that either overexpress human A53T α-synuclein or have mutations within the vesicular...

article

Modeling the C. elegans nematode and its environment using a particle system

Mauno Rönkkö, Garry Wong

Journal of Theoretical Biology > 2008 > 253 > 2 > 316-322

A particle system, as understood in computer science, is a novel technique for modeling living organisms in their environment. Such particle systems have traditionally been used for modeling the complex dynamics of fluids and gases. In the present study, a particle system was devised to model the movement and feeding behavior of the nematode Caenorhabditis elegans in three different virtual environments:...

article

Whole genome microarray analysis of C. elegans rrf-3 and eri-1 mutants

Suvi Asikainen, Markus Storvik, Merja Lakso, Garry Wong

FEBS Letters > 2007 > 581 > 26 > 5050-5054

We performed genome wide gene expression analysis on L4 stage Caenorhabditis elegans rrf-3(pk1426) and eri-1(mg366) mutant strains to study the effects caused by loss of their encoded proteins, which are required for the accumulation of endogenous secondary siRNAs. Mutant rrf-3 and eri-1 strains exhibited 72 transcripts that were co-over-expressed and 4 transcripts co-under-expressed (>2-fold,...

article

Increased lifespan in transgenic Caenorhabditis elegans overexpressing human α-synuclein

Suvi Vartiainen, Vuokko Aarnio, Merja Lakso, Garry Wong

Experimental Gerontology > 2006 > 41 > 9 > 871-876

α-Synuclein is a short 14-kDa protein found in pathological lesions of age-related neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, and multiple system atrophy. Its overexpression in transgenic mice, rats, Drosophila melanogaster, and Caenorhabditis elegans recapitulates many of the pathologic features observed in human Parkinson’s disease including loss of dopaminergic...

article

Identification of gene expression changes in transgenic C. elegans overexpressing human α-synuclein

Suvi Vartiainen, Petri Pehkonen, Merja Lakso, Richard Nass, more

Neurobiology of Disease > 2006 > 22 > 3 > 477-486

α-Synuclein containing cellular inclusions are a hallmark of Parkinson Disease, Lewy Body Dementia, and Multiple System Atrophy. A genome wide expression screen was performed in C. elegans overexpressing both wild-type and A53T human α-synuclein. 433 genes were up- and 67 genes down-regulated by statistical and fold change (> or <2) criteria. Gene ontology (GO) categories within the regulated...

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Search results for: Garry Wong

Identification of potential blood biomarkers for Parkinson’s disease by gene expression and DNA methylation data integration analysis

Human amyloid β peptide and tau co-expression impairs behavior and causes specific gene expression changes in Caenorhabditis elegans

Novel tumor biomarker based on isomiR expression profiles

Chronic MeHg exposure modifies the histone H3K4me3 epigenetic landscape in Caenorhabditis elegans

RNA‐Seq Reveals Acute Manganese Exposure Increases Endoplasmic Reticulum Related and Lipocalin mRNAs in Caenorhabditis elegans

Evolutionary conservation and function of the human embryonic stem cell specific miR-302/367 cluster

Transcriptional profiling reveals differential expression of a neuropeptide-like protein and pseudogenes in aryl hydrocarbon receptor-1 mutant Caenorhabditis elegans

Methylmercury exposure increases lipocalin related (lpr) and decreases activated in blocked unfolded protein response (abu) genes and specific miRNAs in Caenorhabditis elegans

Chronic Ethanol Exposure Increases Cytochrome P‐450 and Decreases Activated in Blocked Unfolded Protein Response Gene Family Transcripts in Caenorhabditis elegans

Radiation-induced genomic instability in Caenorhabditis elegans

Trans fat diet causes decreased brood size and shortened lifespan in Caenorhabditis elegans delta‐6‐desaturase mutant fat‐3

Determination of endocannabinoids in nematodes and human brain tissue by liquid chromatography electrospray ionization tandem mass spectrometry

Overexpression of SUMO perturbs the growth and development of Caenorhabditis elegans

Genes regulated in MPTP-treated macaques and human Parkinson's disease suggest a common signature in prefrontal cortex

Fatty acid composition and gene expression profiles are altered in aryl hydrocarbon receptor-1 mutant Caenorhabditis elegans

Global microRNA Expression Profiling of Caenorhabditis elegans Parkinson's Disease Models

Modeling the C. elegans nematode and its environment using a particle system

Whole genome microarray analysis of C. elegans rrf-3 and eri-1 mutants

Increased lifespan in transgenic Caenorhabditis elegans overexpressing human α-synuclein

Identification of gene expression changes in transgenic C. elegans overexpressing human α-synuclein

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