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A simple, selective and sensitive ion-pair liquid chromatography method was described for the determination of metformin in human plasma. Plasma samples (200 μL) were deproteinated with 10% (v/v) perchloric acid followed by LC analysis using a Symmetry C18 column and a mobile phase of 29% acetonitrile—71% 2 mM sodium dodecyl sulfate (with 350 μL 0.1 M HCl) operated at a flow rate of 1.5 mL min−1....
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