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In a red bacterial strain SF238 belonging to Sporosarcina aquimarina, a C30 carotenoid biosynthetic pathway was identified. It has been reconstructed by analysis of intermediates that accumulate in two different pigment mutants. It starts with the synthesis of 4,4′-diapophytoene and proceeds with its desaturation to 4,4′-diapolycopene, which is then oxidized to 4,4′-diapolycopene-4,4′-dioate. Using...
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