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The initial kinetics for the syntheses of N-(benzyloxycarbonyl)-l-alanyl-l-phenylalanine methyl ester (ZAPM) and N-(benzyloxycarbonyl)-l-aspartyl-l-phenylalanine methyl ester (ZDPM) in an aqueous/organic biphasic system, using free thermolysin were elucidated, both experimentally and theoretically. As model organic solvents, ethyl acetate and tert-amyl alcohol were used. The substrate concentration...
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