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Scope of the present paper is to develop non-resonant Raman microspectroscopic mapping techniques for single cells studies in order to identify subcellular structures by their spectral signatures. Human lung fibroblast cells were fixed in formalin and stored in buffer in order to prevent morphological and chemical changes during data acquisition. Raman maps were recorded with 1μm step size at 785nm...
Objective of our work is the development of new diagnostic methods for detection of tissue states (e.g. tumors, necrosis) in vivo. Therefore, it is important to understand the spectral features of pure individual components of tissues, that means cells and subcellular components. Raman spectroscopy has promising potential as an analytical tool for clinical applications because it can probe the chemical...
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