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The araA gene encoding l-arabinose isomerase (l-AI) from the acidophilus bacterium Lactobacillus fermentum CGMCC2921 was cloned and over-expressed in Escherichia coli. The open reading frame of the l-AI consisted of 1425 nucleotides encoding 474 amino acid residues. The molecular mass of the enzyme was estimated to be approximately 53kDa on SDS–PAGE. The purified recombinant enzyme showed maximum...
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