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Pythium indigoferae and Pythium irregulare, identified based on morphological and physiological characteristics, were isolated from necrotic roots, crown tissues and the rhizosphere of apple trees in Tunisia from 23 apple orchards in spring and autumn 2007–2009. The virulence assays on excised twigs, using different Pythium species isolated demonstrated that these oomycetes were pathogenic on the...
Traditional methods alone often make it difficult to identify isolates to species level. Armillaria and Cylindrocladium are examples where new technologies could achieve this quickly and reliably. Rapid identification of pathogens is of great importance in their control, especially when dealing with quarantine organisms where detection of pathogens at early stages is critical.
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