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Brewery spent grains (BSG), one of the by-products of brewery production, were used for Escherichia coli growth and hydrogen (H2) production. The dilute acid and alkali pretreatment methods were used to hydrolyze the rough lignocellulose structure, and optimal conditions for the BSG hydrolysate (BSGH) preparation were developed. E. coli BW25113 wild type strain and hydrogenase (Hyd)-negative mutants...
Hydrogen gas (H2) production was studied in bacteria cultured at different pH conditions, and on different carbon substrates. H2 production by Escherichia coli was first studied during glycerol fermentation when external formate (10 mM) was supplemented. Changes in H2 production rate (VH2) were determined when in the assays glycerol and/or formate were supplemented at pH 7.5 and pH 6.5, using single...
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