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Fruit ripening is a complex, genetically programmed process involving the action of critical transcription factors (TFs). Despite the established significance of dehydration‐responsive element binding (DREB) TFs in plant abiotic stress responses, the involvement of DREBs in fruit ripening is yet to be determined.
Here, we identified four genes encoding ripening‐regulated DREB TFs in banana (Musa acuminata...
•The hairpin‐based RNA interference (RNAi) technique plays an important role in exploring gene function in plants. Although there are several methods for making hairpin RNA (hpRNA) constructs, these methods usually need multiple relatively laborious, time‐consuming or high‐cost cloning steps. Here we describe a one‐step, zero‐background ligation‐independent cloning (OZ‐LIC) method for making intron‐containing...
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