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Transglutaminase (TGase, EC 2.3.2.13) from a Streptomyces hygroscopicus strain isolated from soil was purified from culture broth by ethanol precipitation, followed by successive chromatographies on CM-cellulose and Sephadex G-75 columns with a yield and purification-fold of 21.1% and 30%, respectively. The enzyme’s molecular weight was estimated as 38,000Da by sodium dodecyl sulfate polyacrylamide...
Effect of some additives on the thermal stability of a new microbial transglugaminase from Streptomyces hygroscopicus WSH03-13, and the stabilization of MTG by spray drying were studied and compared with freeze-drying method. It was observed that MTG was not stable at 50°C (loss of over 50% after 30min of incubation) and became more sensitive to heat in the presence of ethanol. The addition of carbohydrates...
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