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The objective of the study was to evaluate the effectiveness of various methods of RNA extraction from feces of diarrhoeic piglets to diagnose rotaviral infections by the polymerase chain reaction (PCR). The most effective among the four methods of extraction appeared to be the extraction of RNA with quanidine thiocyanate followed by purification on glass powder. This method also enabled an effective...
The purpose of the studies was to determine the rate of rotaviral infection in piglets with the signs of diarrhoea. Samples of faeces were collected from 531 piglets from selected farms, aged 2—8 weeks. The examinations were performed by the ELISA method. The rotaviral antigen was found in piglets from 10 out of 11 farms under study. The frequency of virus occurrence ranged within 6—52% and was highest...
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