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The paper considers some major problems of adapting the multi-detector method for three-dimensional (3D) imaging of wet bio-medical samples in Variable Pressure/Environmental Scanning Electron Microscope (VP/ESEM). The described method pertains to “single-view techniques”, which to create the 3D surface model utilise a sequence of 2D SEM images captured from a single view point (along the electron...
A computer programme for numerical modelling of electron flow in vacuum instruments is presented. The programme allows to simulate trajectories of charged particles in both high and low vacuum of the order of tens milibars. It combines a commercially available packet SIMION 3D v.7.0 destined for tracing trajectories of charged particles in electric and magnetic fields, and a Monte Carlo programme...
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