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To study the molecular mechanism of epididymal protease inhibitor (Eppin) modulating the liquafication of semen. Human semenogelin cDNA (nucleotides 82-849) and Eppin cDNA (nucleotides 70-423) were generated by PCR and cloned into pET-100D/TOPO. Recombinant Eppin and Sg were produced by BL21 (DE3). The association of Eppin with Sg was studied by far-western and radioautography. In vitro the digestion...
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