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The integration of sample pretreatment remains one of the hurdles towards a rapid, automated micro total analytical system (µ-TAS) for real samples. In this paper, a modular design, which was used for sample preparation, has been developed as the polydimethylsiloxane (PDMS) millifluidic chips with channels at a millimeter level. Multiple functional units, including extraction, filtration, mixing and...
This work describes a novel electrochemiluminescence aptasensor for highly sensitive detection of platelet-derived growth factor BB (PDGF-BB) using aptamer functionalized CdS quantum dots–polyamidoamine as probe (CdS QDs–PAMAM–Apt). CdS QDs–PAMAM nanocomposites were synthesized by one-pot synthesis in methanol. The prepared nanocomposites were linked with the NH2–aptamer 2 (Apt2) of PDGF-BB to form...
A sensitive and label-free electrochemiluminescence (ECL) aptasensor for the detection of adenosine triphosphate (ATP) was successfully designed using host–guest recognition between a metallocyclodextrin complex, i.e., tris(bipyridine)ruthenium(II)-β-cyclodextrin [tris(bpyRu)-β-CD], and an ATP-binding aptamer. In the protocol, the NH 2 -terminated aptamer was immobilized on a glassy carbon...
In this study, a rapid and accurate ultra-fast liquid chromatography–tandem quadrupole mass spectrometry (UFLC–MS/MS) method combined with dispersive micro-solid-phase extraction (d-µ-SPE) using a core–shell nanoring amino-functionalized magnetic polymer (CS-NR-MP) was established and validated to determine trace dicyandiamide (DCD) in powdered milk. The developed d-µ-SPE cleanup procedure can dramatically...
A novel, simple and sensitive method was developed for the simultaneous determination of eight phenolic environmental estrogens in blood by using the dispersive micro-solid-phase extraction (d-µ-SPE) procedure combined with ultra-fast liquid chromatography–tandem quadrupole mass spectrometry (UFLC–MS/MS). The excellent nanomaterials tetraethylenepentamine-functionalized magnetic polymer was used as...
We have developed a microfluidic method to construct an array on a circular disk for staining assays. In this method, convenient centrifugal liquid pumping has been achieved within the spiral microchannels by disk rotation or spinning. Moreover, the liquids flowing in spiral channels effectively interact with the along-channel intercepted cell trapping holes. Live cells were encapsulated in wet low-melting...
Salicylic acid (SA) is a biological substance that acts as a phytohormone and plays an important role in signal transduction in plants. It is important to accurately and sensitively detect SA levels. A gold electrode modified with copper nanoparticles was used to assay the electrocatalytic oxidation of salicylic acid. It was found that the electrochemical behavior of salicylic acid was greatly improved...
Valone has a chronic and toxic anticoagulant rodenticide that has widely used in China and has resulted in some accidental and intentional intoxication in recent years. The literature reported so far lacks sensitive and selective method for the confirmation of valone. The purpose of this study was to establish a novel assay for the identification and quantification of valone in serum by ion chromatography–electrospray...
Certain characteristic fragmentations of tryptamines (indoleethylamine) and phenethylamines are described. Based on the GC–EI/MS, LC–ESI/MS and MALDI/TOFMS, the mass fragmentations of 13 standard compounds, including α-methyltryptamine (AMT), N,N-dimethyltryptamine (DMT), 5-methoxy-α-methyltryptamine (5-MeO-AMT), N,N-diethyltryptamine (DET), N,N-dipropyltryptamine (DPT), N,N-dibutyltryptamine (DBT),...
Carbon nanotube powder microelectrodes (CNTPMEs) were used to study the anodic oxidation of hydrazine at Carbon nanotube (CNT)-the novel carbon material. It was found that the electrochemical behaviours were greatly improved at CNTs, indicating that the anodic oxidation could be catalyzed at CNTs. The kinetics parameters of this process were calculated, the heterogeneous electron transfer rate constant...
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