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Three‐photon microscopy excited at the 1700‐nm window enables deep‐tissue penetration. However, the refractive indices of commonly used immersion oils, and the resultant pulse broadening are not known, preventing imaging optimization. Here, we demonstrate detailed characterization of the refractive index, pulse broadening and distortion for excitation pulses at this window for commonly used immersion...
Multiphoton microscopy (MPM) enables noninvasive optical imaging into the deep tissue in animal models in vivo. Recently, 1700 nm has been demonstrated as a promising excitation window for deeper penetration. Signal depletion is currently the limiting factor for imaging depth at the 1700-nm window in MPM. As a result, efficient signal detection is an effective means to further boost imaging depth...
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