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In this study, a combination of double bond equivalence (DBE) distributions and hydrogen–deuterium exchange mass spectrometry (HDX-MS) was applied to elucidate the chemical structures of nitrogen-containing compounds in polar fractions prepared by medium pressure liquid chromatography (MPLC). The N1 and N1S1 class compounds in the fraction eluted with 100% toluene (Polar1) were determined to have...
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