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This paper reports on utilizing a hydrogel for surface immobilization of various reagents in a microfluidic dielectric sensor for blood coagulation monitoring. The top inner surface of the microfluidic sensor is coated with either pure or bio-active polyethylene glycol (PEG), which is immobilized by photo-crosslinking using ultraviolet (UV) light. The coagulation time of healthy human whole blood...
This brief reports on the analysis of a circuit model for human whole blood in a microfluidic dielectric sensor. The sensor employs a novel, 3-D, parallel-plate, capacitive sensing structure with a floating electrode integrated onto a microfluidic channel with 9 ${ \mu }\text{L}$ of sample volume. A circuit model is developed that accurately captures the characteristics of the capacitive double-layer...
This paper describes the design and construct of a fully biocompatible, microfluidic, dielectric sensor targeted at monitoring human whole blood coagulation at the point-of-care (POC). The sensor assembly procedure involves using sputtered electrodes in a microfluidic channel with a physiologically relevant height of 50μm to create a three-dimensional (3D), parallel-plate, capacitive sensing area...
Dielectric spectroscopy (DS) is a powerful monitoring technique that can extract important molecular and cellular properties from a variety of biological materials. DS measurements on human whole blood can reveal a wealth of physiologic information, primarily from the dispersion region that arises from interfacial polarization of red blood cells (RBCs) [1]. Specifically, the use of DS to monitor the...
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