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To construct recombinant adeno-associated virus co-expressing human vascular epithelial growth factor 165(hVEGF 165 ) and bone morphogenetic protein 7(hBMP 7 ), measure the virus titer and verify the recombination. The AAV helper-free system was used as basis to generate recombinant AAV. The IRES sequence of plasmid pIRES was cut down and subcloned into ITR/ MCS containing vector...
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